Part:BBa_K195411:Design

pTetR+RBS+PelB+F10scFv+C-IgAP+Term

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1107
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 134
  Illegal NgoMIV site found at 1118
  Illegal NgoMIV site found at 1743
  Illegal AgeI site found at 1518
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 999
  

Design Notes

The PelB+scFv+C-IgAP didn't have any scar,so we used PCR to In-fusion.

Source

This part is composed of pTetR(BBa_R0040),RBS(BBa_B0034),PelB(BBa_J32015),F10scFv(BBa_K195400),C-IgAP(BBa_K195406) and terminator (BBa_B0015)